METHODS OF DIFFERENTIATING PATHOGENS
GRANULOMATOSES
- Acid-resistant Microorganisms:
Families: Nocardiaceae, Legionellaceae,
Mycobacteriaceae,Cryptosporiidae, etc.
The genus Mycobacterium:
Includes more than 130 species of mycobacteria
Mycobacterium species:
Pathogenic: Mycobacterium tuberculosis complex
Conditionally pathogenic: M. aviumintracellulare
Saprophytic: M. smegmatis, M. phlei, etc.
Bacterioscopy with staining by CN or fluorescent
leprae;
M. fortuitum;
immunohistochemistry
Real-time PCR in FPAC research complex
- Objectives:
1.detection of the pathogen.
2.* Species identification
3.* Determination of MBT number
4.* Determination of MBT resistance profile to
5.Аntituberculosis drugs
-Types of material:
Tissue material - vestibular or autopsy
Paraffin blocks- Limitations:
1.DNA fragment to be determined within
2.100-150 paranucleotides.
3.Tissue fixation in gentle fixatives
4."Age" of the block not more than 2 years
