4.4. Instrument response to the analyte

The instrument response to ethyl alcohol over the range of 0.5 to 2 times the target concentration was determined from multiple injections of analytical standards. The response was linear with a slope of 30.6.

Table 4.4. Instrument Response
× target concn µg/standard	0.5× 12065	1.0× 23750	2.0× 46835
ISTD corrected areas	372840 381097 379231 381919 371549 377760	747373 721669 754169 730028 743159 745198	1477996 1435538 1431093 1437428 1428796 1447001
mean	377399.3	740266.0	1442975.3

Figure 4.4. Calibration curve for ethyl alcohol.

4.5. Storage data

Thirty-six samples were collected using sampling tubes containing Anasorb 747 (Lot 645) over 2 days (18 samples each day) from controlled test atmospheres containing an average of 1979 ppm ethyl alcohol. The high level of ethyl alcohol (twice the target concentration) was used so that a sufficient number of samples to perform a storage test could be collected in a single day's run. Each sample was collected for 2 h at 0.05 L/min. The average relative humidity of the controlled test atmospheres was 75% at 26°C. Six samples (3 each day) were analyzed immediately after collection. Fifteen samples were stored in a refrigerator at about 5°C, and 15 different samples were stored in the dark at about 23°C. Every few days, 3 samples from each group were selected and analyzed. The recovery of ethyl alcohol from samples stored at ambient temperature remained above 102%.

Table 4.5. Storage Tests
days of amb. storage	% recovery (ambient)			days of ref. storage		% recovery (refrigerated)
0	105.0	105.4	102.4	0		102.6	104.4	100.3
3	104.2	105.4	103.3	2		104.3	105.7	102.9
7	103.4	101.6	100.7	6		102.5	105.5	102.5
10	104.2	102.9	103.4	9		104.8	103.3	100.4
14	101.3	102.7	101.6	13		106.0	104.9	103.6
16	104.6	102.3	101.7	15		103.4	106.9	102.9
Figure 4.5.1. Ambient temperature storage test.					Figure 4.5.2. Refrigerated temperature storage test.