Основы биологии_Лекция-5_Макеев (2013)

&!' : # % 3D 1D

/$

&!'

$- /-

$%( $ ( $ ( 100

+ $ ( $

Wasserman, W.W. and Sandelin, A. (2004)

, 5, 2010

5 ( ( $% # " &!'-

(

4 % $ &!'

$% /-# % (SELEX)

% ( (Protein binding assays, PBA) 2 + (EMSA, gel shift)

$ &!' I

$# # % (ChIP-, -chip, -seq, -pet, -exo…)

, 5, 2010

! , %-. + 4

- 1 . .

	In vivo/	%/	&		!
	in vitro	-		(	'
		% &!'
ChIP	In vivo	%	500 -	500 –	! %
{Seq, Pet, Chip}			5000	50 000	% (
					(Piggy back)
%	in vivo	.	28	20-50	% (
	((				%
	()
SELEX, RSS	In vitro	.	20	20-50	$ $
PBA	In vitro	.	60	>10000
$(	In vitro	%	20-100	5-30
					. .

& % ( , $( % /

% 4, ( 0 (

, 5, 2010

4 &!'

1 &!', % (-.

$% ( +

+ ( &!'

CCCGACCA

AAATTTAT

(piggy back)

CCCGACTA

! ( $ &!',

/ (

( % (-. (

4, + + . 4

-% $ &!',

% (-. / 4.

Wasserman, W.W. and Sandelin, A. (2004)

, 5, 2010

+ ( 4?

…AGACTTCCACGAACTTTA…

+ ( &!' (rSNP, remodeling)

+ (

$% (

Suzuki, Forrest (2009)

Nature genetics

, 5, 2010

$# # % (ChIP)

# % ChIP-chip

ChIP-seq

2 ( ChIP-PET

/$ ChIP-exo

: http://www.tigr.org/

&: ( % ( % in vivo

!: $% % ( "

~300 , - ChIP-exo

, 5, 2010

* (

BLAST Basic Local Alignment Search Tool blast.ncbi.nlm.nih.gov

nucleotide blast

Search a nucleotide database using a nucleotide query protein blast

Search protein database using a protein query blastx

Search protein database using a translated nucleotide query tblastn

Search translated nucleotide database using a protein query tblastx

Search translated nucleotide database using a translated nucleotide query

, 5, 2010