Biomedical EPR Part-B Methodology Instrumentation and Dynamics - Sandra R. Eaton

Contents of Previous Volumes

455

VOLUME 19

Chapter 1

Distance Measurements by CW and Pulsed EPR

Sandra S. Eaton and Gareth R. Eaton

Chapter 2

Relaxation Times of Organic Radicals and Transition Metal Ions

Sandra S. Eaton and Gareth R. Eaton

Chapter 3

Structural Information from CW-EPR Spectra of Dipolar Coupled Nitroxide Spin Labels

Eric J. Hustedt and Albert H. Beth

Chapter 4

Determination of Protein Folds and Conformational Dynamics using Spin-Labeling EPR Spectroscopy

Hassane S. Mchaourab and Eduardo Perozo

Chapter 5

EPR Spectroscopic Ruler: The Deconvolution Method and Its

Applications

Wenzhong Xiao and Yeon-Kyun Shin

Chapter 6

TOAC: The Rigid Nitroxide Side Chain

Joseph C. McNulty and Glenn L. Millhauser

Chapter 7

Depth of Immersion of Paramagnetic Centers in Biological Systems

Gertz I. Likhtenshtein

Chapter 8

Determination of Distances Based on and Effects

Sandra S. Eaton and Gareth R. Eaton

456	Contents of Previous Volumes

Chapter 9

Double-Quantum ESR and Distance Measurements

Petr P. Borbat and Jack H. Freed

Chapter 10

“2+1” Pulse Sequence as Applied for Distance and Spatial Distribution Measurements of Paramagnetic Centers

A. Raitsimring

Chapter 11

Double Electron–Electron Resonance

Gunnar Jeschke, Martin Pannier, and Hans W. Spiess

Chapter 12

Electron Paramagnetic Resonance Distance Measurements in

Photosynthetic Reaction Centers

K. V. Lakshmi and Gary W. Brudvig

Chapter 13

Photo-Induced Radical Pairs Investigated using Out-of-Phase Electron Spin Echo

Sergei A. Dzuba and Arnold J. Hoff

VOLUME 20

Chapter 1

Transverse Relaxation Optimized Spectroscopy

Konstantin V. Pervushin

Chapter 2

Segmental Isotopic Labeling: Prospects for a New Tool to Study the Structure-Function Relationships in Multi-Domain Proteins

Jennifer J. Ottesen, Ulrich K. Blaschke, David Cowburn, and Tom W. Muir

Contents of Previous Volumes

457

Chapter 3

Characterization of Inter-Domain Orientations in Solution Using the NMR Relaxation Approach

David Fushman and David Cowburn

Chapter 4

Global Fold Determination of Large Proteins using Site-Directed Spin Labeling

John Battiste, John D. Gross, and Gerhard Wagner

Chapter 5

Solid State NMR Studies of Uniformly Isotopically Enriched Proteins

Ann McDermott

Chapter 6

NMR Spectroscopy of Encapsulated Proteins Dissolved in Low Viscosity

Fluids

A. Joshua Wand, Charles R. Babu, Peter F. Flynn, and Mark J. Milton

Chapter 7

Angular Restraints from Residual Dipolar Couplings for Structure Refinement

Christian Griesinger, Jens Meiler, and Wolfgang Peti

Chapter 8

Protein Structure Refinement using Residual Dipolar Couplings

Angela M. Gronenborn

Chapter 9

Hydrogen Bond Scalar Couplings—A New Tool in Biomolecular NMR

Stephan Grzesiek, Florence Cordier, and Andrew Dingley

Chapter 10

NMR Methods for Screening the Binding of Ligands to Proteins— Identification and Characterization of Bioactive Ligands

Thomas Peters, Thorsten Biet, and Lars Herfurth

458	Contents of Previous Volumes

VOLUME 21

Chapter 1

Microwave Engineering Fundamentals and Spectrometer Design

C. J. Bender

Chapter 2

EPR Spectrometers at Frequencies below X-Band

G. R. Eaton and S. S. Eaton

Chapter 3

Frequency Dependence of EPR Sensitivity

G. A. Rinard, R. W. Quine, S. S. Eaton, and G. R. Eaton

Chapter 4

ENDOR Coils and Related Radiofrequency Circuits

C. J. Bender

Chapter 5

The Generation and Detection of Electron Spin Echoes

C. J. Bender

Chapter 6

Convolution-Based Algorithm: From Analysis of Rotational Dynamics to EPR Oximetry and Protein Distance Measurements

A. I. Smirnov and T. I. Smirnova

Chapter 7

1d and 2d Electron Spin Resonance Imaging (ESRI) of Transport and Degradation Processes in Polymers

M. V. Motyakin and S. Schlick

Chapter 8

Peptide-Aggregation and Conformation Properties as Studied by Pulsed Electron-Electron Double Resonance

Y. D. Tsvetkov

Contents of Previous Volumes

459

VOLUME 22

Chapter 1

The Early Years

Oleg Y. Grinberg and Alexander A. Dubinskii

Chapter 2

The Development of High-Field/High-Frequency ESR

Jack H. Freed

Chapter 3

Primary Processes in Photosynthesis: What Do We Learn from HighField EPR Spectroscopy?

Klaus Möbius, Anton Savitsky, and Martin Fuchs

Chapter 4

High Field ESR: Applications to Protein Structure and Dynamics, HF ESR Protein Structure and Dynamics

Keith A. Earle and Alex I. Smirnov

Chapter 5

The Use of Very High Frequency EPR (VHF-EPR) in Studies of Radicals and Metal Sites in Proteins and Small Inorganic Models

Anne-Laure Barra, Astrid Gräslund, and K. Kristoffer Andersson

Chapter 6

Time-Resolved High-Frequency and Multifrequency EPR Studies of SpinCorrelated Radical Pairs in Photosynthetic Reaction Center Proteins

Marion C. Thurnauer, Oleg G. Poluektov, and Gerd Kothe

Chapter 7

Molecular Dynamics of Gd(III) Complexes in Aqueous Solution by HF EPR

Alain Borel, Lothar Helm, and André E. Merbach

Chapter 8

Pulse High-Frequency EPR

Thomas F. Prisner

460	Contents of Previous Volumes

Chapter 9

High-Frequency EPR, ESEEM and ENDOR Studies of Paramagnetic Centers in Single-Crystalline Materials

Edgar J. J. Groenen and Jan Schmidt

Chapter 10

W-Band Pulse ENDOR of Transition Metal Centers in Orientationally Disordered Systems and Single Crystals

Daniella Goldfarb and Vladimir Krymov

Chapter 11

Sample Resonators for Quasioptical EPR

David E. Budil and Keith A. Earle

Chapter 12

The Bruker ELEXSYS E600/680 94 GHz Spectrometer Series

P. Höfer, A. Kamlowski1, G. G. Maresch, D. Schmalbein, and R. T. Weber

Chapter 13

HF EPR Spectra of Spin Labels in Membranes

V. A. Livshits and D. Marsh

Chapter 14

Modern Developments and Prospects in Multi Frequency High Field

EMR

Louis-Claude Brunel, Anna Lisa Maniero, Alexander Angerhofer, Stephen Hill, and J. (Hans) van Tol

VOLUME 23

Section I. James S. Hyde and Biomedical EPR

Chapter 1

Helmut Beinert

Chapter 2

An Incomplete History of Jim Hyde and the EPR Center at MCW

Harold M. Swartz

Contents of Previous Volumes

461

Section II. Biological Free Radicals and Medicine

Chapter 3

Free Radicals and Medicine

Harold M. Swartz, Ronald P. Mason, Neil Hogg,

Balaraman Kalyanaraman, Tadeusz Sarna, Przemyslaw M. Plonka, Mariusz Zareba, P. L. Gutierrez, and Lawrence J. Berliner

Chapter 4

Superoxide Generation from Nitric Oxide Synthase: Role of Cofactors and Protein-Interaction

Jeannette Vásquez-Vivar, Pavel Martásek, and B. Kalyanaraman

Chapter 5

In Vivo Spin Trapping of Free Radical Metabolites of Drugs and Toxic Chemicals Utilizing Ex Vivo Detection

Ronald P. Mason and Maria B. Kadiiska

Chapter 6

Post Processing Strategies in EPR Spin-Trapping Studies

Agnes Keszler and Neil Hogg

Chapter 7

Biophysical Studies of Melanin: Paramagnetic, Ion-Exchange and Redox Properties of Melanin Pigments and Their Photoreactivity

Tadeusz Sarna and Przemyslaw M. Plonka

Chapter 8

Application of Spin Labels To Membrane Bioenergetics: Photosynthetic Systems of Higher Plants

Alexander N. Tikhonov and Witold K. Subczynski

Section III. In Vivo EPR and Physiology

Chapter 9

EPR Spectroscopy of Function In Vivo: Origins, Achievements, And Future Possibilities

Harold M. Swartz and Nadeem Khan

462	Contents of Previous Volumes

Chapter 10

EPR Oximetry in Biological and Model Samples

Witold K. Subczynski and Harold M. Swartz

Chapter 11

In Vivo EPR Imaging

Benjamin B. Williams and Howard J. Halpern

Chapter 12

Time-Domain Radio Frequency EPR Imaging

Sankaran Subramanian and Murali C. Krishna

Section IV. Metals

Chapter 13

Copper Biomolecules in Solution

Riccardo Basosi, Giovanni Della Lunga, and Rebecca Pogni

Chapter 14

Low Frequency EPR of in Proteins

William E. Antholine

Chapter 15

Electron Spin-Echo Envelope Modulation Studies of in Biological

Systems

Michael J. Colaneri and Jack Peisach

Index

250 GHz, 246

2D-ESR, 253

2D-FT-ESR, 257, 262 Accessibility parameter, 272, 276 ACERT, 187, 431

Acylenzyme, 89, 112, 123, 126-131 A/D converters, 217-218

ADP, 104, 110, 111 Alamethicin, 277, 288 Alderman-Grant resonator, 21 Alanine dosimetry, 424 AMBER, 118

Amoxycillin, 116

Angle-selected ENDOR, 96, 101, 104 Anion exchange protein (AE1), 375, 382,

383, 396-398 Annexin XII, 274, 295 Arcing, 24

Ascorbate radical, 54 ATP, 110, 111

per square root watt, 22, 33, 38 circularly polarized component, 34 measurement, 34, 42

Backbone flexibility, 283 Background signal, 22 Bacteriorhodopsin, 273, 282, 376 Balanced structure, 31

Balun, 31 Bandwidth, 40

of resonator, 22, 36 of pulse, 36

Beer, antioxidants, 424 Benzylpenicillin, 116

Bimodal resonator, 43, 172, 175, 242

Birdcage resonator, 21

Bloch equations, 315, 332, 379 Bridged loop-gap resonator, 25,

174, 183

Brownian dynamics, 180, 183 Bruker GMBH, 146, 421, 433 Bruker split-ring resonator, 26, 35,

45

BtuB, 275, 276, 285

102, 104-106 Capacitance, 24, 32

Capacitive coupling, 20, 27, 30 Capacitive gap, 22, 45 acid (Gla),

105, 106 Carboxypeptidase A, 123, 124 Cavity, rectangular, 19

See also, resonator, Cellular retinol binding protein

(CRBP), 275, 282 Cephaloglycin, 113, 114, 121 Cephalosporin, 114, 116, 121, 128

spin-labeled, 113-117, 121, 122, 134

CHARMM, 118

Cholestane, 254

Cholesterol, 186 112, 118, 126 CIDEP, 245

CIDNP, 245

Circular dichroism, 106 Coax-to-waveguide transition, 28 Colicin E1, 273, 276 Conductivity, 38

463

464	Index
Conformer, 109, 112, 115-120	coupling, 64, 65
Coordination geometry, 90, 103, 106	Diels-Alder catalyst, 105
Copper, 44	Diffusion, anisotropic, 330
copper-coated Teflon, 25	Diffusion coefficient, 324, 327
tellurium-copper alloy, 44	translational, 343, 347, 350
Correlation spectroscopy, 2D, 179	Digital detection, 199-221, 410,
COSY, 179, 259	424, 434
Coupling coefficient, 38	Digital signal processor (DSP), 219
Coupling loop, 28	Dipolar coupling, 174, 224, 227,
Coupling technique, 30	228
Coupling to waveguide, 29	Dipolar frequency, 226, 229
Cross correlation, 207	Dipolar relaxation, 343
Cross relaxation, 5, 149, 166, 228, 255, 256,	Dipole-dipole interaction, 98, 99,
263	224, 226, 290
Crossed-coil resonator, 21, 432	paramagnetic enhancement, 343,
CROX, chromium oxalate, 272, 273, 278	347
Cryocrystallography, 124	pattern, 25
Cryoenzymology, 90, 124, 125	radiator, 20
Cryogenic operation, 26	Diphtheria toxin, 276, 277
Cryokinetics, 123-127, 132	Direct digital synthesizer, 215
Cryoprotectant, 229, 230	Disorder matrix, 168
Cryosolvent, 124-126, 131-133	Distance, see also DEER, 181
Crystallin, 275, 296	between spin labels, 224, 290-
Cytochrome c, 66-71, 76-79, 286	298
Cytochrome c oxidase, 57-59, 277, 325, 396	distributions, 228
CTPO, 57, 182	electron-electron, 169, 223
	electron-nuclear, 89, 91, 93, 98,
Dap (diaminopropionic acid) spin label, 289	99, 103, 113-115, 118, 128,
DD-transpeptidase, 126, 127	129, 133, 134
Dead time, 37, 40, 41, 43, 227	DMPC liposomes, 184
DEER (PELDOR), 168, 169, 170, 175, 176,	DMPO-OH, 59-61
181, 187, 225, 227, 228, 231, 293, 431	Double quantum EPR, double
3-pulse DEER, 225, 227, 230	quantum coherence 169, 224,
4-pulse DEER, 175, 225, 227	225, 228, 230, 293
Density functional theory, 82	Doubly-tuned resonator, 172
Dephasing mechanism, 233	Dynamic frequency shifts, 241
Design equations for LGR, 31	Dynamics, snapshot, 248
Detection,
superheterodyne, 201, 203	Echo detected EPR, 7
homodyne, 201, 204, 208	Echo envelope modulation, 7
quadrature, 201	Echo intensity, 233
Deuterium, 102, 114, 128, 129, 132	Eddy current, 36
Dewar, 42, 44	EED (electron-electron dipolar)
Dielectric constant, 96, 97, 106, 117-119	relaxation, 174
Dielectric resonator, 19, 45, 62-65, 72, 174	EGF receptor, 375, 382, 396
65	Eigenfunction expansion, 376, 386