- •Saparbekova a.A., Aimenova Zh.E.
- •Composers: Saparbekova a.A., Aimenova Zh.E.
- •Content
- •Introduction
- •Safety measures in microbiological laboratory
- •Laboratory work №1 Methods of microscopic examination of microorganisms. Microscope components.
- •Electron microscopy
- •Scanning probe microscopy
- •Types of microscopes
- •Optical microscope
- •Lighting techniques
- •Optical configurations of microscope
- •Components of microscope
- •Laboratory work № 2 Nutrient media. Preparation of ware and media for sterilisation
- •Maintenance of Aseptic Environment
- •Laboratory work №3 Microorganisms morphology and methods of its study
- •Laboratory work № 4 Structure of bacteria and yeasts
- •In the history…
- •Blood agar plates (bap)
- •Chocolate agar (choc)
- •Sabouraud agar
- •Hay infusion agar
- •Potato dextrose agar
- •Inoculation of Culture Media
- •Importance of Using “Sterile Technique”
- •Inoculating the Agar Slant
- •Inoculating the nb
- •Inoculating the na butt
- •Laboratory work № 5 Cultivation of microorganisms
- •Laboratory work № 6
- •Isolation of accumulative pure cultures of bacteria
- •Common Methods of isolation of pure culture
- •Streak Plate Method
- •Various methods of streaking
- •Laboratory work № 7 Control for cultivation. Antimicrobial factors.
- •Laboratory work № 8 Microflora of microbial synthesis products
- •List of recommended literature
- •Saparbekova a.A., Aimenova Zh.E. Microbiology and virology
Ф.__________
Saparbekova a.A., Aimenova Zh.E.
METHODICAL INSTRUCTIONS
TO LABORATORY CLASSES
ON MICROBIOLOGY AND VIROLOGY
Shymkent, 2012 y.
MINISTRY OF SCIENCE AND EDUCATION OF REPUBLIC OF KAZAKHSTAN
M.AUEZOV SOUTH KAZAKHSTAN STATE UNIVERSITY
«Biotechnology» department
Methodical instructions to laboratory classes
On Microbiology and virology
For students on specialty 5B070100 Biotechnology
Study form: full time and external
Shymkent, 2012 y.
UDK _____
BBK _____
М ____
Composers: Saparbekova a.A., Aimenova Zh.E.
Methodical instructions to laboratory classes on “Microbiology and virology”- Shymkent: M.Auezov SKSU, 2012.- _____pages.
Methodical instructions are made according to requirements of the curriculum and the program of discipline Microbiology and virology and includes all necessary data on performance of laboratory researches of a course.
Methodical instructions are made for students on specialty 5B070100 – Biotechnology.
Reviewers: _____________________________________________________
_____________________________________________________
The methodical instructions is recommended to the edition by Methodical council of M.Auezov SKSU, Minutes № __ from « » ____________2012 y.
© M.Auezov South Kazakhstan State University, 2012
Content
The themes of the laboratory works |
pages |
Introduction |
|
The rules of working in microbiological laboratory |
|
1. Methods of microscopic examination of microorganisms. Microscope components. |
|
2. Nutrient media. Sterilization of nutrient medium and of the ware. |
|
3. Microorganisms morphology. |
|
4. Structure of bacteria and yeasts. |
|
5. Cultivation of microorganisms. |
|
6. Accumulative pure cultures. |
|
7. Control for the cultivation. Antimicrobial factors. Microbes-antagonists. |
|
8. Microflora of the products of microbial synthesis. |
|
Further reading |
|