- •Saparbekova a.A., Aimenova Zh.E.
- •Composers: Saparbekova a.A., Aimenova Zh.E.
- •Content
- •Introduction
- •Safety measures in microbiological laboratory
- •Laboratory work №1 Methods of microscopic examination of microorganisms. Microscope components.
- •Electron microscopy
- •Scanning probe microscopy
- •Types of microscopes
- •Optical microscope
- •Lighting techniques
- •Optical configurations of microscope
- •Components of microscope
- •Laboratory work № 2 Nutrient media. Preparation of ware and media for sterilisation
- •Maintenance of Aseptic Environment
- •Laboratory work №3 Microorganisms morphology and methods of its study
- •Laboratory work № 4 Structure of bacteria and yeasts
- •In the history…
- •Blood agar plates (bap)
- •Chocolate agar (choc)
- •Sabouraud agar
- •Hay infusion agar
- •Potato dextrose agar
- •Inoculation of Culture Media
- •Importance of Using “Sterile Technique”
- •Inoculating the Agar Slant
- •Inoculating the nb
- •Inoculating the na butt
- •Laboratory work № 5 Cultivation of microorganisms
- •Laboratory work № 6
- •Isolation of accumulative pure cultures of bacteria
- •Common Methods of isolation of pure culture
- •Streak Plate Method
- •Various methods of streaking
- •Laboratory work № 7 Control for cultivation. Antimicrobial factors.
- •Laboratory work № 8 Microflora of microbial synthesis products
- •List of recommended literature
- •Saparbekova a.A., Aimenova Zh.E. Microbiology and virology
List of recommended literature
Basic literature:
1. Advances in Biochemical Engineering/Biotechnology, Vol.1-, A.Fiechter (ed.), Springer-Verlag, Berlin, Germany Bergey’s Manual of Systematic Bacteriology, Vol.1-4, J.G.Holt, Editor-in-Chief, Williams and Wilkins, Baltimore, MD, USA, 1984-1989
2. Biology of Microorganisms, 6th Edition, T D. Brock & M.T Madigan, Prentice-Hall International Inc., New Jersey, USA 1991
3. Biotechnology, 2nd, Completely Revised Edition, Vol. 1-12, H.J.Rehm & G.Reed (series editors), VCH, Weinheim, Germany, 1993
4. Biotechnology, Ullmann’s Encyclopedia of Industrial Chemistry, 5th Edition, Vol. A4, H. G. Schlegel, H.W. Doelle, A. Fiechter, H.Yamada, S. Shimizu, 107-170 VCH, Weinheim, Germany, 1985
5.CRC Handbook Series in Nutrition and Food, Vol. lll, Section G., M. Rechtigl Jr., Editor-inChief, Media and Media Interactions, CRC Press Inc., Cleveland, OH, USA, 1978
Additional literature:
1. Current Protocols in Molecular Biology, F M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J.G. Seidman, J. A. Smith, and K. Struhl, Looseleaf Service, Wiley-Liss, New York, USA
2. Encyclopedia of Microbiology, Vol. 1-4, J. Lederberg, Editor-in-Chief, Academic Press Inc., Orlando, FL, USA, 1992
3. Escherichia coli and Salmonella typhimurium, J. L. Ingraham et al. (eds.), American Society for Microbiology, Washington, D.C., USA, 1987
4. Frontiers of Microbiology, G. C. Walker & D. Kaiser (eds.), American Society for Microbiology, Washington, D.C., USA, 1993
5. Industrial Microorganisms, R. H. Baltz & G. D. Hegemann (eds.), American Society for Microbiology, Washington, D.C., USA, 1993
Saparbekova a.A., Aimenova Zh.E. Microbiology and virology
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